Concentrations of iron correlate with the extent of protein, but not
lipid, oxidation in advanced human atherosclerotic lesions.
Stanley N, Stadler N, Woods AA, Bannon PG, Davies MJ
Free Radic Biol Med. 2006 May 1; 40(9): 1636-43
Previous studies have provided compelling evidence for the presence of
oxidized proteins and lipids in advanced human atherosclerotic lesions.
The catalyst responsible for such oxidation is unknown and
controversial. We have previously provided evidence for elevated levels
of iron in lesions. In this study we hypothesized that if iron ions
catalyzed protein and lipid oxidation in the artery wall, then there
should be a positive correlation between these parameters. Iron
concentrations in ex vivo healthy human arteries and advanced carotid
lesions were quantified by electron paramagnetic resonance
spectroscopy. Four specific side-chain oxidation products of proteins,
and the lipid oxidation products 7-ketocholesterol and cholesterol
ester alcohols and hydroperoxides, were quantified by HPLC in the same
samples used for the iron measurements. Parent amino acids,
cholesterol, and cholesterol esters were also quantified. Statistically
elevated levels of iron, cholesterol, cholesterol esters,
7-ketocholesterol, and cholesterol ester alcohols and hydroperoxides
were detected in advanced lesions compared with healthy control tissue.
Iron levels correlated positively and strongly with all four markers of
protein oxidation, but not with either marker of lipid oxidation. These
data support the hypothesis that elevated levels of iron contribute to
the extent of protein, but not lipid, oxidation in advanced human
lesions.
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