Inhibition of lipoxygenase also significantly inhibit bladder cancer growth

Discussion in 'Health and medical' started by Roger, Feb 27, 2004.

  1. Roger

    Roger Guest

    That's pancreatic, bladder, and prostate cancer that is inhibited by lipoxygenase inhibitors (see
    the previous two abstracts I just posted). Might work for most if not all cancers.


    J Urol. 2003 Nov;170(5):1994-9.

    Expression of lipoxygenase in human bladder carcinoma and growth inhibition by its inhibitors.

    Yoshimura R, Matsuyama M, Tsuchida K, Kawahito Y, Sano H, Nakatani T. Department of Urology, Osaka
    City University Medical School, 1-4-3 Asahi-machi, Abenoku, Osaka 545-8585, Japan

    PURPOSE: The metabolism of arachidonic acid by the cyclooxygenase or lipoxygenase pathway generates
    eicosanoids, which have been implicated in the pathogenesis of various human diseases, including
    cancer. They are now believed to have important roles in tumor promotion, progression and
    metastasis. The involvement of lipoxygenase expression and function in tumor growth and metastasis
    has been reported in human tumor cell lines.

    MATERIALS AND METHODS: The expression of 5 and 12-lipoxygenase in patients with bladder tumor and
    chronic cystitis, and in normal bladder tissues was examined. We also examined the effects of their
    inhibitors on cell proliferation in a bladder cancer cell line. The expression of 5 and 12-
    lipoxygenase protein was detected by immunohistochemistry. The effects of lipoxygenase inhibitors on
    bladder cancer cell growth were examined by MTT (3-[4,5-dimethylthiazol-2-thiazolyl]-2,5-
    diphenyltetrazolium bromide) assay, while Hoechst (Sigma Chemical Co., St. Louis, Missouri) staining
    was used to determine whether lipoxygenase inhibitors induce apoptosis.

    RESULTS: While slight 5 and 12-lipoxygenase expression was detected in chronic cystitis and normal
    bladder tissues, marked 5 and 12-lipoxygenase expression was detected in bladder cancer tissues.
    Lipoxygenase inhibitors caused marked inhibition of bladder cancer cells in a concentration and time
    dependent manner. Cells treated with lipoxygenase inhibitors showed chromatin condensation, cellular
    shrinkage, small membrane bound bodies (apoptotic bodies) and cytoplasmic condensation.

    CONCLUSIONS: Lipoxygenase is induced in bladder cancer. Results suggest that lipoxygenase inhibitors
    may mediate potent antiproliferative effects against bladder cancer cells. Thus, lipoxygenase may
    become a new target in the treatment of bladder tumors.