I
Jpn J Ophthalmol. 2005 Sep-Oct;49(5):355-62. Links
Vitamins inhibit oxidant-induced apoptosis of corneal endothelial
cells.
Serbecic N, Beutelspacher SC.
Department of Ophthalmology, SMZ-Ost, Donauspital, Vienna, Austria.
PURPOSE: To determine the effects of vitamins A, C, and E
supplementation on lipid peroxidation and apoptosis in corneal
endothelial cells. METHODS: Murine corneal endothelial cells were
maintained in tissue culture medium supplemented with free iron ions,
known to lead to increased lipid peroxidation. The concentration of
antioxidative vitamins (ascorbic acid, tocopherol, and retinoic acid)
in the cells and supernatant was determined using reversed-phase
high-performance liquid chromatography. Apoptosis was assessed by
quantification of caspase-3-like activity, using annexin-V/propidium
iodide stains for flow cytometry. Lipid peroxidation was assessed using
the malondialdehyde method. Supplementation of antioxidative vitamins
was tested in the setting of apoptosis. RESULTS: Increasing levels of
free iron led to a rapid loss of antioxidative vitamins in the
supernatant and corneal endothelial cells. This was correlated with
rising levels of malondialdehyde and increased apoptosis.
Supplementation with ascorbic acid or alpha-tocopherol alone was not
sufficient to prevent lipid peroxidation in the cells, whereas a
combination of vitamins C and E was able to do so. In contrast,
supplementation with vitamin A alone significantly reduced oxidative
stress and apoptosis. CONCLUSIONS: We present an in vitro model to test
the direct influence of vitamin supplementation on corneal endothelial
cells with regard to lipid peroxidation and apoptosis. We show that
supplementation with antioxidative vitamins of corneal endothelial
cells significantly prevents the generation of free-radical injury,
lipid peroxidation, and consequent apoptosis. Jpn J Ophthalmol
2005;49:355-362 (c) Japanese Ophthalmological Society 2005.
PMID: 16187034 [PubMed - in process]
--------------------------------------------------------------------------------
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Vitamins inhibit oxidant-induced apoptosis of corneal endothelial
cells.
Serbecic N, Beutelspacher SC.
Department of Ophthalmology, SMZ-Ost, Donauspital, Vienna, Austria.
PURPOSE: To determine the effects of vitamins A, C, and E
supplementation on lipid peroxidation and apoptosis in corneal
endothelial cells. METHODS: Murine corneal endothelial cells were
maintained in tissue culture medium supplemented with free iron ions,
known to lead to increased lipid peroxidation. The concentration of
antioxidative vitamins (ascorbic acid, tocopherol, and retinoic acid)
in the cells and supernatant was determined using reversed-phase
high-performance liquid chromatography. Apoptosis was assessed by
quantification of caspase-3-like activity, using annexin-V/propidium
iodide stains for flow cytometry. Lipid peroxidation was assessed using
the malondialdehyde method. Supplementation of antioxidative vitamins
was tested in the setting of apoptosis. RESULTS: Increasing levels of
free iron led to a rapid loss of antioxidative vitamins in the
supernatant and corneal endothelial cells. This was correlated with
rising levels of malondialdehyde and increased apoptosis.
Supplementation with ascorbic acid or alpha-tocopherol alone was not
sufficient to prevent lipid peroxidation in the cells, whereas a
combination of vitamins C and E was able to do so. In contrast,
supplementation with vitamin A alone significantly reduced oxidative
stress and apoptosis. CONCLUSIONS: We present an in vitro model to test
the direct influence of vitamin supplementation on corneal endothelial
cells with regard to lipid peroxidation and apoptosis. We show that
supplementation with antioxidative vitamins of corneal endothelial
cells significantly prevents the generation of free-radical injury,
lipid peroxidation, and consequent apoptosis. Jpn J Ophthalmol
2005;49:355-362 (c) Japanese Ophthalmological Society 2005.
PMID: 16187034 [PubMed - in process]
--------------------------------------------------------------------------------
Who loves ya.
Tom
Jesus Was A Vegetarian!
http://jesuswasavegetarian.7h.com
Man Is A Herbivore!
http://pages.ivillage.com/ironjustice/manisaherbivore
DEAD PEOPLE WALKING
http://pages.ivillage.com/ironjustice/deadpeoplewalking