Iron / oxidation in the eyes / antioxidants

Discussion in 'Food and nutrition' started by [email protected], Sep 28, 2005.

  1. Jpn J Ophthalmol. 2005 Sep-Oct;49(5):355-62. Links


    Vitamins inhibit oxidant-induced apoptosis of corneal endothelial
    cells.

    Serbecic N, Beutelspacher SC.

    Department of Ophthalmology, SMZ-Ost, Donauspital, Vienna, Austria.

    PURPOSE: To determine the effects of vitamins A, C, and E
    supplementation on lipid peroxidation and apoptosis in corneal
    endothelial cells. METHODS: Murine corneal endothelial cells were
    maintained in tissue culture medium supplemented with free iron ions,
    known to lead to increased lipid peroxidation. The concentration of
    antioxidative vitamins (ascorbic acid, tocopherol, and retinoic acid)
    in the cells and supernatant was determined using reversed-phase
    high-performance liquid chromatography. Apoptosis was assessed by
    quantification of caspase-3-like activity, using annexin-V/propidium
    iodide stains for flow cytometry. Lipid peroxidation was assessed using
    the malondialdehyde method. Supplementation of antioxidative vitamins
    was tested in the setting of apoptosis. RESULTS: Increasing levels of
    free iron led to a rapid loss of antioxidative vitamins in the
    supernatant and corneal endothelial cells. This was correlated with
    rising levels of malondialdehyde and increased apoptosis.
    Supplementation with ascorbic acid or alpha-tocopherol alone was not
    sufficient to prevent lipid peroxidation in the cells, whereas a
    combination of vitamins C and E was able to do so. In contrast,
    supplementation with vitamin A alone significantly reduced oxidative
    stress and apoptosis. CONCLUSIONS: We present an in vitro model to test
    the direct influence of vitamin supplementation on corneal endothelial
    cells with regard to lipid peroxidation and apoptosis. We show that
    supplementation with antioxidative vitamins of corneal endothelial
    cells significantly prevents the generation of free-radical injury,
    lipid peroxidation, and consequent apoptosis. Jpn J Ophthalmol
    2005;49:355-362 (c) Japanese Ophthalmological Society 2005.

    PMID: 16187034 [PubMed - in process]

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  2. Ma¢k

    Ma¢k Guest

    On 28 Sep 2005 03:42:48 -0700, "[email protected]"
    <[email protected]> Huffed and Puffed the following into the madness
    of usenet:


    I bet you had nightmares the first time you watched the X-men.

    Magneto is coming for you.....
     
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