D
Doe
Guest
This would give some credence to the theory of targeting of iron as an antiinflammatory. Since rutin
is a potent iron binder.
<<snip>> The present study revealed that rutin possesses anti-inflammatory properties. <<snip>>
Rev Med Chir Soc Med Nat Iasi. 2002 Oct-Dec;106(4):801-5. Related Articles, Links
[In Process Citation]
[Article in Romanian]
Gherase F, Pavelescu MD, Stanescu U, Grigorescu E.
Facultatea de Farmacie, Disciplina de Farmacodinamie, Universitatea de Medicina si Farmacie Gr.T.
Popa Iasi.
In the present investigation we have evaluated the potential existence of analgesic activity of the
residue of tincture and of the crude lipophilic (AC 2, AC 4) and hydrophilic portions (AC 1, AC 3)
of infusion and decoction obtained from flower heads of Achillea collina J. Becker ex Reichenb. Acid
acetic induced writhing and vascular permeability test was performed by the method of Whittle.
Comparison was made with indomethacin, morphine and rutin, as references drugs. The mice were dosed
orally with samples. AC 2 induced a potent inhibition (47.23%; p < 0.05) of the nociceptive response
after oral administration of 250 mg/kg--a dose ten folds higher than the dose of indomethacin. AC 3,
at a dose of 5000 mg/kg (p.o.) inhibited with 47.6% (p < .001) and 19.8% (p < 0.05) the increase in
peritoneal vascular permeability caused by 0.5% acetic acid. Relative unexpected, morphine (1.14
mg/kg, p.o.), an opioid analgesic, also produced a significant reduction of the leakage
(41.2%, p < 0.01). This finding is consistent with the reports in which the role of the endogenous
opiate system in the physiological response to injury/inflammation, mainly controlling fluid
leakage into the interstitial space, is sustained.
PMID: 14974233 [PubMed - in process]
--------------------------------------------------------------------------
------
Exp Toxicol Pathol. 2003 Mar;54(4):313-8. Related Articles, Links
Anti-inflammatory effect of rutin on rat paw oedema, and on neutrophils chemotaxis and
degranulation.
Selloum L, Bouriche H, Tigrine C, Boudoukha C.
Laboratory of Applied Biochemistry, Department of Biology, Faculty of Sciences, University of Ferhat
ABBAS, 19000 Setif, Algeria. [email protected]
BACKGROUND: Rutin, a natural flavone derivative, is known for its pharmacological properties. We
have previously reported that this flavonol exerted a potent inhibitory effect on respiratory burst
of fMet-Leu-Phe-stimulated neutrophils, as well as on phosphoinositide 3-kinase gamma activity in a
cell free system. In the present study, the anti-inflammatory effect of rutin was investigated in
vivo and in vitro. METHODS: rutin or aspirin (100 mg/kg, body weight) were given orally to rats 1
hour before paw oedema induction, using lambda-carrageenan 1%. The rat paw volume was measured by
mean of plethysmometer, initially and during 6 hours. The chemotaxis of neutrophils towards 10(-7) M
fMet-Leu-Phe was performed using 48-well chemotaxis chamber. Neutrophils that migrated through 5
microm pore size polycarbonate filter, in presence or in absence of rutin, were counted
microscopically. Elastase exocytosis of either phorbol 12-myristate 13-acetate or fMet-Leu-
Phe/cytochalasin B-stimulated neutrophils was assessed in absence or in presence of rutin using the
synthetic substrate N-Suc-Ala-Ala-Ala-p-nitroanilide. The absorbance of released p-nitroaniline was
measured at 405 nm using microplate reader. RESULTS: The maximal swelling in placebo group was
observed at 5 hours, after lambda-carrageenan injection. Oral administration of rutin reduced rat
paw swelling starting 2 hours after lambda-carrageenan injection. Rutin reduced significantly (p <
0.05) and in a dose-dependant manner the polymorphonuclear neutrophils chemotaxis to fMet-Leu-Phe.
Furthermore, elastase exocytosis, induced by both stimuli, was partially inhibited by rutin up to 25
microM. CONCLUSION: The present study revealed that rutin possesses anti-inflammatory properties.
PMID: 12710715 [PubMed - indexed for MEDLINE]
--------------------------------------------------------------------------
------
Who loves ya. Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore!
http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING
http://pages.ivillage.com/ironjustice/deadpeoplewalking
is a potent iron binder.
<<snip>> The present study revealed that rutin possesses anti-inflammatory properties. <<snip>>
Rev Med Chir Soc Med Nat Iasi. 2002 Oct-Dec;106(4):801-5. Related Articles, Links
[In Process Citation]
[Article in Romanian]
Gherase F, Pavelescu MD, Stanescu U, Grigorescu E.
Facultatea de Farmacie, Disciplina de Farmacodinamie, Universitatea de Medicina si Farmacie Gr.T.
Popa Iasi.
In the present investigation we have evaluated the potential existence of analgesic activity of the
residue of tincture and of the crude lipophilic (AC 2, AC 4) and hydrophilic portions (AC 1, AC 3)
of infusion and decoction obtained from flower heads of Achillea collina J. Becker ex Reichenb. Acid
acetic induced writhing and vascular permeability test was performed by the method of Whittle.
Comparison was made with indomethacin, morphine and rutin, as references drugs. The mice were dosed
orally with samples. AC 2 induced a potent inhibition (47.23%; p < 0.05) of the nociceptive response
after oral administration of 250 mg/kg--a dose ten folds higher than the dose of indomethacin. AC 3,
at a dose of 5000 mg/kg (p.o.) inhibited with 47.6% (p < .001) and 19.8% (p < 0.05) the increase in
peritoneal vascular permeability caused by 0.5% acetic acid. Relative unexpected, morphine (1.14
mg/kg, p.o.), an opioid analgesic, also produced a significant reduction of the leakage
(41.2%, p < 0.01). This finding is consistent with the reports in which the role of the endogenous
opiate system in the physiological response to injury/inflammation, mainly controlling fluid
leakage into the interstitial space, is sustained.
PMID: 14974233 [PubMed - in process]
--------------------------------------------------------------------------
------
Exp Toxicol Pathol. 2003 Mar;54(4):313-8. Related Articles, Links
Anti-inflammatory effect of rutin on rat paw oedema, and on neutrophils chemotaxis and
degranulation.
Selloum L, Bouriche H, Tigrine C, Boudoukha C.
Laboratory of Applied Biochemistry, Department of Biology, Faculty of Sciences, University of Ferhat
ABBAS, 19000 Setif, Algeria. [email protected]
BACKGROUND: Rutin, a natural flavone derivative, is known for its pharmacological properties. We
have previously reported that this flavonol exerted a potent inhibitory effect on respiratory burst
of fMet-Leu-Phe-stimulated neutrophils, as well as on phosphoinositide 3-kinase gamma activity in a
cell free system. In the present study, the anti-inflammatory effect of rutin was investigated in
vivo and in vitro. METHODS: rutin or aspirin (100 mg/kg, body weight) were given orally to rats 1
hour before paw oedema induction, using lambda-carrageenan 1%. The rat paw volume was measured by
mean of plethysmometer, initially and during 6 hours. The chemotaxis of neutrophils towards 10(-7) M
fMet-Leu-Phe was performed using 48-well chemotaxis chamber. Neutrophils that migrated through 5
microm pore size polycarbonate filter, in presence or in absence of rutin, were counted
microscopically. Elastase exocytosis of either phorbol 12-myristate 13-acetate or fMet-Leu-
Phe/cytochalasin B-stimulated neutrophils was assessed in absence or in presence of rutin using the
synthetic substrate N-Suc-Ala-Ala-Ala-p-nitroanilide. The absorbance of released p-nitroaniline was
measured at 405 nm using microplate reader. RESULTS: The maximal swelling in placebo group was
observed at 5 hours, after lambda-carrageenan injection. Oral administration of rutin reduced rat
paw swelling starting 2 hours after lambda-carrageenan injection. Rutin reduced significantly (p <
0.05) and in a dose-dependant manner the polymorphonuclear neutrophils chemotaxis to fMet-Leu-Phe.
Furthermore, elastase exocytosis, induced by both stimuli, was partially inhibited by rutin up to 25
microM. CONCLUSION: The present study revealed that rutin possesses anti-inflammatory properties.
PMID: 12710715 [PubMed - indexed for MEDLINE]
--------------------------------------------------------------------------
------
Who loves ya. Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore!
http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING
http://pages.ivillage.com/ironjustice/deadpeoplewalking