Rutin / IBD

Discussion in 'Food and nutrition' started by [email protected], Jan 18, 2005.

  1. Biochem Pharmacol. 2005 Feb 1;69(3):395-406. Epub 2004 Dec 15. Related
    Articles, Links


    Dietary rutin, but not its aglycone quercetin, ameliorates dextran
    sulfate sodium-induced experimental colitis in mice: attenuation of
    pro-inflammatory gene expression.

    Kwon KH, Murakami A, Tanaka T, Ohigashi H.

    Division of Food Science and Biotechnology, Graduate School of
    Agriculture, Kyoto University, Kyoto 606-8502, Japan.

    Oxidative stress has been shown to play a pivotal role in the onset of
    inflammatory bowel disease (IBD) and carcinogenesis. We evaluated the
    effects of two dietary anti-oxidants, rutin and its aglycone quercetin,
    on dextran sulfate sodium (DSS)-induced experimental colitis in mice.
    Female ICR mice were fed a diet containing 0.1% rutin or 0.1% quercetin
    for 2 weeks, and given 5% DSS in drinking water during the second week
    to induce colitis. We also examined the dose-dependency of rutin and
    quercetin (0.01% and 0.001% each) as well as their therapeutic
    efficacy, which was evaluated following DSS administration, on
    DSS-induced colitis. The protein level of interleukin (IL)-1beta in
    both colonic mucosa and peritoneal macrophages was quantified by
    enzyme-linked immunosorbent assay. Further, mRNA expression levels of
    IL-1beta, tumor necrosis factor-alpha, IL-6, granulocyte
    macrophage-colony stimulating factor, inducible nitric oxide synthase,
    and cyclooxygenase (COX)-1 and COX-2 in colonic mucosa were determined
    by reverse transcription-polymerase chain reaction. A diet containing
    0.1% rutin, but not quercetin, attenuated DSS-induced body weight loss
    and shortening of the colorectum (P<0.01 and <0.05, respectively), and
    dramatically improved colitis histological scores. Further, DSS-induced
    increases in colonic mucosal IL-1beta levels were blunted significantly
    in rutin-, but not quercetin-, fed mice (P<0.01), while dietary rutin
    attenuated the expressions of IL-1beta and IL-6 mRNA in colonic mucosa
    (each, P<0.01). As for dose dependency, 0.01%, but not 0.001%, dietary
    rutin significantly reduced mucosal IL-1beta levels (P<0.01). Notably,
    a 0.1% rutin diet given 3 days after DSS treatment significantly
    suppressed both colorectal shortening and IL-1beta production (P<0.05
    and <0.01, respectively). Dietary rutin ameliorates DSS-induced
    colitis, presumably by suppressing the induction of pro-inflammatory
    cytokines. Our results suggest that rutin may be useful for the
    prevention and treatment of IBD and colorectal carcinogenesis via
    attenuation of pro-inflammatory cytokine production.

    PMID: 15652231 [PubMed - as supplied by publisher]

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