General fitness, health and nutrition · Public discussion

Stem cells and marrow

ironjustice · 2007-04-03T20:30:39+00:00

Would / does .. ? .. phlebotomy / venesection / bloodletting ..ALSO .. 'create' .. in INCREASED numbers .. stem cells ALONG with new red blood cells .. ? So .. would the fact an 'anemic' person would have bone marrow proliferation at a higher 'renewal' rate of someone who is .. polycythemic .. ? .. because a person in iron REreplete state would be creating red blood cells FROM .. strictly .. iron / haematinic .. http://tinyurl.com/24nquv Whereas .. one creating red blood cells FROM the .. marrow .. marked by INCREASED erythropoietin PRODUCTION due to lack of iron / haematinic, would be perpetuating the self renewal of ALL cells related TO.. this 'marrow' .. process .. ? Therefore INCLUDING .. stem cells .. And on the flip side .. since increased iron seems to self renew red blood cells / erythrocytosis would this lead to a .. decreased .. amount of stem cells .. As evidenced by the low .. erythropoietin .. STILL creating red blood cells .. evidencing PLENTY of .. haematinic / iron .. 'on board' .. because a low erythropoietin is a **sign** OF .. plenty of haematinic / iron / blood **forming** .. elements. Primitive Adult Stem Cells Found In Peripheral Blood Main Category: Stem Cell Research News Article Date: 18 Apr 2006 - 0:00 PDT Biology / Biochemistry Moraga Biotechnology Corporation, an adult stem cell company based in Los Angeles, California, announces the discovery of its proprietary Blastomere-Like Stem Cells (BLSCs) circulating in the peripheral blood of mammals. The Company's scientists found that these adult stem cells were able to differentiate into most tissues and organs of the body. The Company's scientists have developed a proprietary and cost- effective method for isolating and purifying large numbers of its primitive embryonic- like stem cells from the blood without ex vivo expansion. Moraga recently announced that its BLSCs were found in large numbers throughout the body. The Company believes this new discovery is a major breakthrough for finding large numbers of primitive adult stem cells circulating in the blood. Moraga's chief executive, Dr. John F. Wong, noted that the ability to isolate these primitive embryonic-like stem cells in peripheral blood allows the Company to enter the stem cell banking business in the near future. With this new method for isolating stem cells, the Company will be able to bank an individual's stem cells by simply withdrawing a sample of their blood. The Company intends in the very near future to establish processing centers to which a donor's blood sample is shipped; the blood is processed; and, the stem cells are isolated. The BLSCs are then stored at the center for future therapeutic and diagnostic use. Dr. Wong further stated that "by circumventing the expensive process of isolating and expanding the stem cells outside the body, the Company can now travel down a shorter path towards developing a near-term solution for autologous stem cell-based therapies, from which its BLSCs may be used for treating various forms of diseases such as heart attacks, Parkinson's disease and stroke." Moraga Biotechnology Corporation http://www.moragabiotech.com -------------------------------------------------------------------- Definitions of haematinic on the Web: hematinic: a medicine that increases the hemoglobin content of the blood; used to treat iron-deficiency anemia wordnet.princeton.edu/perl/webwn -------------------------------------------------------------------- Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk

Started by ironjustice · 3 April 2007 · Last activity 27 May 2007 · 5 posts · 131 views

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Original section: General fitness, health and nutrition
Published: 3 April 2007
Last activity: 27 May 2007
Original author: ironjustice
Posts: 5
Discussion status: Public discussion
Total views: 131
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Original post
ironjustice
Archived post

3 April 2007, 20:30 UTC #1

Would / does .. ? .. phlebotomy / venesection / bloodletting ..ALSO ..
'create' .. in INCREASED numbers .. stem cells ALONG with new red
blood cells .. ?
So .. would the fact an 'anemic' person would have bone marrow
proliferation at a higher 'renewal' rate of someone who is ..
polycythemic .. ? .. because a person in iron REreplete state would be
creating red blood cells FROM .. strictly .. iron / haematinic ..
http://tinyurl.com/24nquv
Whereas .. one creating red blood cells FROM the .. marrow .. marked
by INCREASED erythropoietin PRODUCTION due to lack of iron /
haematinic, would be perpetuating the self renewal of ALL cells
related TO.. this 'marrow' .. process .. ?
Therefore INCLUDING .. stem cells ..
And on the flip side .. since increased iron seems to self renew red
blood cells / erythrocytosis would this lead to a .. decreased ..
amount of stem cells ..
As evidenced by the low .. erythropoietin .. STILL creating red blood
cells .. evidencing PLENTY of .. haematinic / iron .. 'on board' ..
because a low erythropoietin is a **sign** OF .. plenty of
haematinic / iron / blood **forming** .. elements.
Primitive Adult Stem Cells Found In Peripheral Blood
Main Category: Stem Cell Research News
Article Date: 18 Apr 2006 - 0:00 PDT
Biology / Biochemistry
Moraga Biotechnology Corporation, an adult stem cell company based in
Los Angeles, California, announces the discovery of its proprietary
Blastomere-Like Stem Cells (BLSCs) circulating in the peripheral blood
of mammals. The Company's scientists found that these adult stem cells
were able to differentiate into most tissues and organs of the body.
The Company's scientists have developed a proprietary and cost-
effective method for isolating and purifying large numbers of its
primitive embryonic- like stem cells from the blood without ex vivo
expansion. Moraga recently announced that its BLSCs were found in
large numbers throughout the body. The Company believes this new
discovery is a major breakthrough for finding large numbers of
primitive adult stem cells circulating in the blood.
Moraga's chief executive, Dr. John F. Wong, noted that the ability to
isolate these primitive embryonic-like stem cells in peripheral blood
allows the Company to enter the stem cell banking business in the near
future. With this new method for isolating stem cells, the Company
will be able to bank an individual's stem cells by simply withdrawing
a sample of their blood. The Company intends in the very near future
to establish processing centers to which a donor's blood sample is
shipped; the blood is processed; and, the stem cells are isolated. The
BLSCs are then stored at the center for future therapeutic and
diagnostic use. Dr. Wong further stated that "by circumventing the
expensive process of isolating and expanding the stem cells outside
the body, the Company can now travel down a shorter path towards
developing a near-term solution for autologous stem cell-based
therapies, from which its BLSCs may be used for treating various forms
of diseases such as heart attacks, Parkinson's disease and stroke."
Moraga Biotechnology Corporation
http://www.moragabiotech.com
--------------------------------------------------------------------
Definitions of haematinic on the Web:
hematinic: a medicine that increases the hemoglobin content of the
blood; used to treat iron-deficiency anemia
wordnet.princeton.edu/perl/webwn
--------------------------------------------------------------------
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Man Is A Herbivore!
http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

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9 April 2007, 06:41 UTC #2

Iron deprivation blocks multilineage haematopoietic differentiation by
inhibiting induction of p21(WAF1/CIP1)
Authors: Alcantara, Orlando; Boldt, David H.
Source: British Journal of Haematology, Volume 137, Number 3, May
2007, pp. 252-261(10)
Abstract:
Summary
Iron is required for the differentiation of HL-60 cells along the
monocyte lineage in vitro, reflecting a requirement for iron in the
transcriptional induction of the p21(WAF1/CIP1) gene. To determine if
the same requirement holds true for differentiation in other cell
lineages and for primary human CD34+ bone marrow precursor cells, we
induced granulocyte differentiation by treating HL-60 cells with
dimethyl sulphoxide, and erythroid or megakaryocytic differentiation
by treating K562 cells with butyrate or phorbol myristate acetate,
respectively. Nitro blue tetrazolium reduction, expression of haem, or
expression of CD41 was used to assess granulocytic, erythroid, or
megakaryocytic differentiation respectively. Purified CD34+ cells were
cultured with granulocyte/macrophage-colony stimulating factor and
stem cell factor to induce myelomonocytic differentiation. Iron
deprivation was induced by desferrioxamine. p21(WAF1/CIP1) antisense
oligonucleotides were used to inhibit p21 expression. Iron deprivation
blocked p21 induction as judged by real-time polymerase chain reaction
assays. In addition, both iron deprivation and p21 antisense blocked
CD34+ cell differentiation. These observations were not explained by
induction of widespread apoptosis under conditions of iron
deprivation. We concluded that both iron and functional p21(WAF1/CIP1)
are required for in vitro differentiation of human haematopoietic
precursors along multiple cell lineages.
Keywords: iron; haematopoiesis; p21(WAF1/CIP1); CD34+ cells
Document Type: Research article
DOI: 10.1111/j.1365-2141.2007.06549.x
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John Gentile
Archived post

10 April 2007, 01:01 UTC #3

Quoted post said:
We concluded that both iron and functional p21(WAF1/CIP1)
are required for in vitro differentiation of human haematopoietic
precursors along multiple cell lineages.
So iron is NECESSARY to produce the different cells in the blood???
--
John Gentile MS, M(ASCP)
Laboratory Information Mgr.
VA Medical Center
Providence, RI
[email hidden]

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ironjustice
Archived post

10 April 2007, 03:33 UTC #4

Quoted message said:
Quoted message said:
So iron is NECESSARY to produce the different cells in the blood???

<<
You have extramedullary blood production / outside of marrow and you
have marrow blood production.
Sooo .. when one has hemolytic anemia the marrow stores are
commonly .. depleted .. and available markers used seem to point to ..
iron deficiency .. gold standard / no bone MARROW iron.
Sooo .. does the fact the cells are being made ELSEWHERE .. negate the
fact iron is REQUIRED .. ? .. to form .. "ALL human haematopoietic
precursors along multiple cell lineages" .. including therefore ..
stem cells.
Sooo .. the fact the **marrow** is DEVOID .. of .. iron .. DUE TO ..
hemolytic anemia / hemo-lysis .. leads to a decrease in .. normal ..
amounts of .. "ALL human haematopoietic precursors along multiple
cell lineages" .. due TO .. lack of .. ? .. "So iron is NECESSARY to
produce the different cells in the blood???"
<<snip>>
no stainable iron in the bone marrow
<<snip>>
Am J Clin Pathol 1983 Nov;80(5):743-5
Serum ferritin and sequestered stores of body iron.
Rao KR, Ray VH, Patel AR
A 47-year-old man with sickle cell anemia, chronic cor pulmonale,
and
congestive heart failure died following a short illness. A
diagnosis
of iron deficiency was established during life by usually accepted
criteria including a low serum ferritin concentration. Autopsy
showed
no stainable iron in the bone marrow, liver, and the heart. Marked
deposits of iron were seen in the kidneys and the atrophic spleen.
These findings suggest that the serum ferritin concentration may
not
reflect the metabolically sequestered stores of iron in the spleen
and
the kidneys.
PMID: 6195914, UI: 84049241
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Jesus Was A Vegetarian!
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Archived post

27 May 2007, 18:09 UTC #5

ironjustice said:
Quoted message said:
Quoted message said:
So iron is NECESSARY to produce the different cells in the blood???
<<
You haveextramedullaryblood production / outside of marrow and you
have marrow blood production.
Sooo .. when one has hemolytic anemia the marrow stores are
commonly .. depleted .. and available markers used seem to point to ..
iron deficiency .. gold standard / no bone MARROW iron.
Sooo .. does the fact the cells are being made ELSEWHERE .. negate the
fact iron is REQUIRED .. ? .. to form .. "ALL human haematopoietic
precursors along multiple cell lineages" .. including therefore ..
stem cells.
Sooo .. the fact the **marrow** is DEVOID .. of .. iron .. DUE TO ..
hemolytic anemia / hemo-lysis .. leads to a decrease in .. normal ..
amounts of .. "ALL human haematopoietic precursors along multiple
cell lineages" .. due TO .. lack of .. ? .. "So iron is NECESSARY to
produce the different cells in the blood???"
<<snip>>
no stainable iron in the bone marrow
<<snip>>
Am J Clin Pathol 1983 Nov;80(5):743-5
Serum ferritin and sequestered stores of body iron.
Rao KR, Ray VH, Patel AR
A 47-year-old man with sickle cell anemia, chronic cor pulmonale,
and
congestive heart failure died following a short illness. A
diagnosis
of iron deficiency was established during life by usually accepted
criteria including a low serum ferritin concentration. Autopsy
showed
no stainable iron in the bone marrow, liver, and the heart. Marked
deposits of iron were seen in the kidneys and the atrophic spleen.
These findings suggest that the serum ferritin concentration may
not
reflect the metabolically sequestered stores of iron in the spleen
and
the kidneys.
PMID: 6195914, UI: 84049241
Who loves ya.
Tom
Jesus Was A Vegetarian!http://jesuswasavegetarian.7h.com
Man Is A Herbivore!http://tinyurl.com/a3cc3
DEAD PEOPLE WALKINGhttp://tinyurl.com/zk9fk
Quoted message said:
Quoted message said:
You have extramedullaryblood production / outside of marrow and you

have marrow blood production.
Sooo .. when one has hemolytic anemia the marrow stores are
commonly .. depleted .. and available markers used seem to point
to ..
iron deficiency .. gold standard / no bone MARROW iron.
Sooo .. does the fact the cells are being made ELSEWHERE .. negate
the
fact iron is REQUIRED .. ? .. to form .. "ALL human haematopoietic
precursors along multiple cell lineages" .. including therefore ..
stem cells.
Sooo .. the fact the **marrow** is DEVOID .. of .. iron .. DUE TO ..
hemolytic anemia / hemo-lysis .. leads to a decrease in .. normal ..
amounts of .. "ALL human haematopoietic precursors along multiple
cell lineages" .. due TO .. lack of .. ? .. "So iron is NECESSARY to
produce the different cells in the blood???"<<
I believe the above hypothesis .. has been given credence by this
article .. ?
Curing the patient .. and so does .. **stem cell** production NOW ..
increase .. too .. ?
<<snip>>
resulting in long-lasting transfusion-free survival
<<snip>>
Correction of anemia in a transfusion-dependent patient with primary
myelofibrosis receiving iron chelation therapy with deferasirox
(Exjade®, ICL670)
Authors: Di Tucci, Anna Angela1; Murru, Roberta1; Alberti, Daniele2;
Rabault, Bertrand2; Deplano, Simona1; Angelucci, Emanuele1
Source: European Journal of Haematology, Volume 78, Number 6, June
2007, pp. 540-542(3)
Publisher: Blackwell Publishing
Abstract:
Transfusional iron overload in patients with chronic anemias can
result in multiple organ failure. Experience in the management of
iron
overload in patients with myelodysplastic syndromes is limited, as
many do not receive chelation therapy due to short-life expectancy
and
the difficulties associated with the administration of the current
reference standard chelator, deferoxamine. There have, however, been
some reports of reduced transfusion requirement associated with
chelation therapy in patients with myelodysplastic syndromes and
myelofibrosis. Here, we discuss a patient with primary myelofibrosis
and related transfusion-dependent anemia who received chelation
therapy with the once-daily oral iron chelator, deferasirox. In
addition to the reduced iron levels, the patient demonstrated an
unexpected reduction in blood transfusion requirement, ultimately
resulting in long-lasting transfusion-free survival.
Keywords: myelofibrosis; iron overload; chelation
Document Type: Research article
DOI: 10.1111/j.1600-0609.2007.00840.x
Affiliations: 1: Unità Operativa Ematologia e Centro Trapianti
Midollo
Osseo, Ospedale Oncologico `Armando Businco', Cagliari, Italy 2:
Novartis Pharma AG, Basel, Switzerland
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Tom
Jesus Was A Vegetarian!
http://jesuswasavegetarian.7h.com
Man Is A Herbivore!
http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

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