>Subject: Viruses Transmitted To People Through Meat
>From:
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>Date: 3/21/2004 12:59 PM Mountain Standard Time
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http://news.bbc.co.uk/1/hi/health/3520968.stm
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Microb Pathog. 2004 May;36(5):263-71. Related
Articles, Links
Heme-iron increases levels of AP65-mediated adherence by
Trichomonas vaginalis.
Alderete JF, Nguyen J, Mundodi V, Lehker MW.
Department of Microbiology, University of Texas Health
Science Center at San Antonio, 7703 Floyd Curl Dr., MC 7758,
San Antonio TX 78284-7758, USA.
Trichomonas vaginalis is a protozoan responsible for the
number one, non-viral
mediate adherence to vaginal epithelial cells (VECs). Iron
increases growth of trichomonads and synthesis and surface
placement of adhesins. We observed by immunofluorescence
using monoclonal antibody (mAb) 12G4 the placement of AP65
on surfaces of trichomonads supplemented with hemoglobin or
hemin as a source of iron. We, therefore, tested the
hypothesis that heme-bound iron is an alternative source of
iron important to trichomonal growth and regulation of
expression of the adhesin genes. Here we show that the
inhibition of parasite growth by the iron chelator 2,2-
dipyridal is rescued by hemoglobin or hemin, but not
protoporphyrin IX. Importantly, trichomonads grown in iron-
limiting medium supplemented with free iron, hemoglobin and
hemin had elevated levels of ap65 transcript that were 12.6-
, 12.3- and 9.2-fold higher, respectively, than low-iron
organisms, as determined by RT-PCR. Similarly, the amounts
of AP65 were 8.9-, 11.2-, and 4.8-fold higher in parasites
grown in free iron, hemoglobin and hemin, respectively, than
organisms in low-iron medium. The heme-iron-regulated AP65
increased adherence of parasites to immortalized VECs. Not
surprisingly, parasites pretreated with anti-AP65 serum IgG
had decreased adherence compared to organisms incubated with
prebleed serum IgG. These data illustrate that heme-bound
iron is a source of iron similar to lactoferrin. This work
extends our findings about the multiple sources of iron for
regulating virulence genes of T. vaginalis.
PMID: 15043861 [PubMed - in process]
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