Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
Effect of an acute dose of ethanol on lipid peroxidation in rats: action of
vitamin E.
Jordao AA Jr, Chiarello PG, Arantes MR, Meirelles MS, Vannucchi H.
Postgraduation in Food Science, Faculty of Pharmaceutical Sciences, University
of Sao Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirao Preto-SP, Brazil.
Free radical generation is an important step in the pathogenesis of
ethanol-associated liver injury. Administration of ethanol induces an increase
in lipid peroxidation both by enhancing the production of oxygen reactive
species and by decreasing the levels of endogenous antioxidants. This work
focuses on the generation of free radicals provoked by an acute ethanol dose in
rats, and the role of different dietary levels of vitamin E. The objective of
this investigation was to study the effect of three different dietary levels of
vitamin E (deficient, control and supplemented with 20 times higher levels) on
plasma and liver lipid peroxidation (assayed by TBARS), vitamin E in plasma and
liver, and hepatic glutathione concentration, in rats receiving the different
diets. The animals were submitted to an acute dose of ethanol (5 g/kg body
weight) administered by gavage at the end of an experimental 4 week period and
were sacrificed at 0, 2, 4, 8 and 24 h after ethanol administration. Dietary
vitamin E caused a dose-dependent increase in liver and plasma concentration of
the vitamin, but ethanol administration decreased hepatic vitamin E in all
groups. TBARS concentrations were higher in liver of rats that received the
deficient diet, independent of ethanol, however, liver TBARS concentrations
were low in control and supplemented groups, but increased with ethanol
ingestion. Glutathione levels were lowered by ethanol administration in all
groups, in different times, but recovered to this original level in 24 h time.
In conclusion, vitamin E deficiency alone induces liver lipid peroxidation in
rats, acute administration of ethanol affect vitamin E and GSH level and
maintenance of adequate or higher vitamin E levels acts as a protective factor
against free radical generation.
PMID: 14871588 [PubMed - in process]
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