Low-fat diet increases oxidized LDL

On Fri, 19 Mar 2004 16:11:44 +0200, Matti Narkia <[email hidden]>

Quoted message said:

In the recent study

Silaste ML, Rantala M, Alfthan G, Aro A, Witztum JL,
Kesaniemi YA, Horkko S. Changes in dietary fat intake alter
plasma levels of oxidized low-density lipoprotein and
lipoprotein(a). Arterioscler Thromb Vasc Biol. 2004 Mar;24(3):498-
503. Epub 2004 Jan 22. <URL:http://atvb.ahajournals.org/cg-
i/content/abstract/24/3/498>

it was found that decrease in dietary fat intake led to
increase of oxidized
LDL.

This study has been commented in the articles

'Healthy' Diet May Increase Bad Cholesterol <URL:http://ww-
w.reuters.com/newsArticle.jhtml?type=healthNews&storyID=46-
00933&section=news> <URL:http://story.news.yahoo.com/news?-
tmpl=story&u=/nm/20040318/hl_nm/cholesterol_diet_dc_1> <UR-
L:http://www.brudirect.com/DailyInfo/News/Archive/Mar04/20-
0304/wn03.htm>

and

"Healthy" Diet May Increase Plasma LDL and Lp(a) in Some
Women <URL:http://www.medscape.com/viewarticle/472074>

Fascinating.

Thanks for the link.

L.

Matti Narkia said:

In the recent study

Silaste ML, Rantala M, Alfthan G, Aro A, Witztum JL,
Kesaniemi YA, Horkko S. Changes in dietary fat intake
alter plasma levels of oxidized low-density lipoprotein
and lipoprotein(a). Arterioscler Thromb Vasc Biol. 2004
Mar;24(3):498-503. Epub 2004 Jan 22. <URL:http://atvb.aha-
journals.org/cgi/content/abstract/24/3/498>

it was found that decrease in dietary fat intake led to
increase of oxidized
LDL.

Their observation that increased intake of fruits and
vegetables did not prevent the increase in oxidized LDL (as
measured by their technique) should lead folks to be
concerned about their methodologies.

Imho, they should have had a "regular" diet control and
possibly even a "high fat" diet control in order to be more
certain about the accuracy of their method of measuring
oxidized LDL (assuming that there is no increase in the
controls, of course).

Servant to the humblest person in the universe,

Andrew

--
Dr. Andrew B. Chung, MD/PhD
Board-Certified Cardiologist
heartmdphd.comheartmdphd.comOpen ↗

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Quoted message said:

Quoted message said:

it was found that decrease in dietary fat intake led to
increase of

oxidized

Quoted message said:

Quoted message said:

LDL.

Their observation that increased intake of fruits and
vegetables did

not prevent the increase in

Quoted message said:

oxidized LDL (as measured by their technique) should lead
folks to be

concerned about their

Actually, it did to some degree. But it was probably
overshadowed by total carbohydrate intake; or perhaps by
increased total calorie intake.

Quoted message said:

Imho, they should have had a "regular" diet control and
possibly even

a "high fat" diet control

Quoted message said:

in order to be more certain about the accuracy of their
method of

measuring oxidized LDL

Apparently they wanted to study benefits of high-carb diet
rich in veggies/fruit vs high-carb diet short of them. This
is something they did not expected at all.

BTW, there was low-fat/normal fat study you suggest
presented one month ago. Results were similiar. Picture is
shaping up... 🙂

Mirek

I think this is significant... Compared with the baseline
levels, the median plasma OxLDL-EO6 increased by 27% (Q1,
Q4: -14, 67) (P<0.01) in response to the low-fat, low-
vegetable diet and by 19% (Q1, Q4: 1, 44) (P<0.01) in
response to the low-fat, high-vegetable diet (Oxidized LDL:A
sandwich chemiluminescent immunoassay using a well
characterized murine monoclonal antibody EO6, which
specifically binds to oxidized phospholipids, was used to
measure plasma oxidized LDL.20,21 First, a monoclonal anti-
apolipoprotein B-100 antibody, MB47, was plated at 5 µg/mL
overnight at 4°C. The plasma samples were diluted to 1:50
and the amount of oxidized phospholipid epitopes was
measured with biotinylated antibody EO6. In parallel wells,
the amount of LDL bound was detected with polyclonal
biotinylated anti-apoB antibody. The results are expressed
as amount of EO6 bound divided by the amount of anti-apoB
bound into the wells, yielding the relative amount of
oxidized phospholipid detected by EO6/apoB-100 (OxLDL-EO6).
This measurement normalizes the oxidized phospholipid
content per apoB-100 particle.)

but the authors do not...

Although the amounts of vegetables, berries, and fruits in
the diet significantly influenced the plasma antioxidant
concentrations, it seemed that they did not affect the
amount of OxLDL-EO6 and Lp(a) in the circulation. Thirty-
seven healthy women were studied. With a 8% difference
attibutable to fruits vegetables and berries alone and
considering the small sample size, wouldn't that weaken the
correlation with fats? I like this study, but wonder if the
authors are having trouble sorting out the priorities. Or is
it just me after a crazy week?

--
Winning against heart attack and stroke
sonoscore.comsonoscore.comOpen ↗

Fri, 19 Mar 2004 20:11:00 +0100 in article
<[email hidden]> "Mirek Fidler" <[email hidden]>

Quoted message said:

BTW, there was low-fat/normal fat study you suggest
presented one month ago. Results were similiar. Picture is
shaping up... 🙂

Do you mean the study

Pelkman CL, Fishell VK, Maddox DH, Pearson TA, Mauger DT,
Kris-Etherton PM. Effects of moderate-fat (from
monounsaturated fat) and low-fat weight-loss diets on the
serum lipid profile in overweight and obese men and women.
Am J Clin Nutr. 2004 Feb;79(2):204-12.
<URL:http://www.ajcn.org/cgi/content/abstract/79/2/204>

which was reported in the articles

Moderate-Fat Diet Better Than Low-Fat Diet at Improving
Cardiovascular Risk Factors
<URL:http://www.medscape.com/viewarticle/468332>

and

Moderate-fat Diet Is Kinder To Heart Than Low-fat Diet,
Study Shows <URL:http://www.buffalo.edu/news/fast-execute.cgi/article-
page.html?article=65630009> <URL:http://www.sciencedaily.co-
m/releases/2004/02/040202070609.htm>

Another related result is:

Dreon DM, Fernstrom HA, Williams PT, Krauss RM. Reduced LDL
particle size in children consuming a very-low-fat diet is
related to parental LDL-subclass patterns. Am J Clin Nutr.
2000 Jun;71(6):1611-6.
<URL:http://www.ajcn.org/cgi/content/full/71/6/1611>

comments in

Extreme low-fat diets can increase heart disease risk for
some <URL:http://www.lbl.gov/Science-
Articles/Archive/extremediet-patternb.html>

More related articles:

Lichtenstein AH. Dietary fat and cardiovascular disease
risk: quantity or quality? J Womens Health (Larchmt). 2003
Mar;12(2):109-14. Review. <ncbi.nlm.nih.goventrOpen ↗
ez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12737709&dop-
t=Abstract> ( tinyurl.comwaojOpen ↗ )

Corr LA, Oliver MF. The low fat/low cholesterol diet is
ineffective. Eur Heart J. 1997 Jan;18(1):18-22.
<URL:http://www.omen.com/corr.html>

Dreon DM, Fernstrom HA, Williams PT, Krauss RM. A very low-
fat diet is not associated with improved lipoprotein
profiles in men with a predominance of large, low-density
lipoproteins. Am J Clin Nutr. 1999 Mar;69(3):411-8
<URL:http://www.ajcn.org/cgi/content/full/69/3/411>

Hu FB, Stampfer MJ, Manson JE, Rimm E, Colditz GA, Rosner
BA, Hennekens CH, Willett WC. Dietary fat intake and the
risk of coronary heart disease in women. N Engl J Med. 1997
Nov 20;337(21):1491-9. PMID: 9366580 [PubMed - indexed for
MEDLINE] ncbi.nlm.nih.govquery.fcgiOpen ↗
Retrieve&db=PubMed&list_uids=9366580&dopt=Abstract

Leddy J, Horvath P, Rowland J, Pendergast D. Effect of a
high or a low fat diet on cardiovascular risk factors in
male and female runners. Med Sci Sports Exerc. 1997 Jan;29(1):17-
25. PMID: 9000152 [PubMed - indexed for MEDLINE] <URL:http:-
//www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Pu-
bMed&list_uids=9000152&dopt=Abstract>

Concepts and Controversies on Diet: Stop Recommending Low-
Fat Diets! Walter C Willett, MD, DrPH Kaiser Permanente
Permanente Journal, September 2003 <URL:http://www.countcar-
bs.com/research/stoprecommendinglowfat.htm>

Fats and Cholesterol - The Good, The Bad, and The Healthy
Diet. Harvard School of Public Health Nutrition Source.
<URL:http://www.hsph.harvard.edu/nutritionsource/fats.html>

The Soft Science of Dietary Fat Gary Taubes Science, Vol
291, Number 5513, Mar 30, 2001 pp. 2536-2545 Winner of the
2001 Science in Society Journalism Award <URL:http://www.ja-
cquigingras.com/pdf/The%20Soft%20Science%20of%20Dietary%20F-
at.pdf> <URL:http://nasw.org/mem-
maint/awards/The%20soft%20science.pdf> <URL:http://www.coun-
tcarbs.com/research/softscience_part1.htm>
<URL:http://timinvermont.com/fat.htm>

--
Matti Narkia

Quoted message said:

Do you mean the study

Pelkman CL, Fishell VK, Maddox DH, Pearson TA, Mauger DT,

Kris-Etherton PM.

Quoted message said:

Effects of moderate-fat (from monounsaturated fat)
and low-fat

weight-loss

Quoted message said:

diets on the serum lipid profile in overweight and
obese men and

women.

Quoted message said:

Am J Clin Nutr. 2004 Feb;79(2):204-12.
<URL:http://www.ajcn.org/cgi/content/abstract/79/2/204>

Yes. Thanks for link.

Mirek

"Sonos" <[email hidden]> wrote in part:

Quoted message said:

I think this is significant... Compared with the baseline
levels, the median plasma OxLDL-EO6 increased by 27% (Q1,
Q4: -14, 67) (P<0.01) in response to the low-fat, low-
vegetable diet and by 19% (Q1, Q4: 1, 44) (P<0.01) in
response to the low-fat, high-vegetable diet (Oxidized
LDL:A sandwich chemiluminescent immunoassay using a well
characterized murine monoclonal antibody EO6, which
specifically binds to oxidized phospholipids, was used to
measure plasma oxidized LDL.20,21 First, a monoclonal anti-
apolipoprotein B-100 antibody, MB47, was plated at 5 µg/mL
overnight at 4°C. The plasma samples were diluted to 1:50
and the amount of oxidized phospholipid epitopes was
measured with biotinylated antibody EO6. In parallel wells,
the amount of LDL bound was detected with polyclonal
biotinylated anti-apoB antibody. The results are expressed
as amount of EO6 bound divided by the amount of anti-apoB
bound into the wells, yielding the relative amount of
oxidized phospholipid detected by EO6/apoB-100 (OxLDL-EO6).
This measurement normalizes the oxidized phospholipid
content per apoB-100 particle.)

but the authors do not...

Although the amounts of vegetables, berries, and fruits in
the diet significantly influenced the plasma antioxidant
concentrations, it seemed that they did not affect the
amount of OxLDL-EO6 and Lp(a) in the circulation. Thirty-
seven healthy women were studied. With a 8% difference
attibutable to fruits vegetables and berries alone and
considering the small sample size, wouldn't that weaken the
correlation with fats? I like this study, but wonder if the
authors are having trouble sorting out the priorities. Or
is it just me after a crazy week?

My guess is that they are a bit trapped by the fact that
there may not be enough statistical power to reject the
hypothesis that the increase in OxLDL-EO6 is the same with
and without the veggies. I would say there is non-
significant (at p=.01) evidence that the veggies worked as
they have been shown to do elsewhere.

Needs more work....
--
Jim Chinnis Warrenton, Virginia, USA

Mirek Fidler said:

Quoted message said:

Quoted message said:

it was found that decrease in dietary fat intake led
to increase of

oxidized

Quoted message said:

Quoted message said:

LDL.

Their observation that increased intake of fruits and
vegetables did

not prevent the increase in

Quoted message said:

oxidized LDL (as measured by their technique) should
lead folks to be

concerned about their

Actually, it did to some degree. But it was probably
overshadowed by total carbohydrate intake; or perhaps by
increased total calorie intake.

Quoted message said:

Imho, they should have had a "regular" diet control and
possibly even

a "high fat" diet control

Quoted message said:

in order to be more certain about the accuracy of their
method of

measuring oxidized LDL

Apparently they wanted to study benefits of high-carb diet
rich in veggies/fruit vs high-carb diet short of them.
This is something they did not expected at all.

BTW, there was low-fat/normal fat study you suggest
presented one month ago. Results were similiar. Picture is
shaping up... 🙂

Mirek

Kindly provide the citation for your latter reference.

Servant to the humblest person in the universe,

Andrew

--
Dr. Andrew B. Chung, MD/PhD
Board-Certified Cardiologist
heartmdphd.comheartmdphd.comOpen ↗

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Who is the humblest person in the universe?
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Mirek Fidler said:

Quoted message said:

Do you mean the study

Pelkman CL, Fishell VK, Maddox DH, Pearson TA,
Mauger DT,

Kris-Etherton PM.

Quoted message said:

Effects of moderate-fat (from monounsaturated fat) and
low-fat

weight-loss

Quoted message said:

diets on the serum lipid profile in overweight and obese
men and

women.

Quoted message said:

Am J Clin Nutr. 2004 Feb;79(2):204-12.
<URL:http://www.ajcn.org/cgi/content/abstract/79/2/204>

Yes. Thanks for link.

Mirek

Your cited reference did not look at oxidized LDL.

Moreover, what is in the parenthesis in the title may be
important.

Imho, the more appropriate control group would have been
moderate fat (with usual mix of saturated fats) diet.

Servant to the humblest person in the universe,

Andrew

--
Dr. Andrew B. Chung, MD/PhD
Board-Certified Cardiologist
heartmdphd.comheartmdphd.comOpen ↗

**
Who is the humblest person in the universe?
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What is all this about?
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Is this spam?
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Quoted message said:

Quoted message said:

Quoted message said:

<URL:http://www.ajcn.org/cgi/content/abstract/79/2/204>

Yes. Thanks for link.

Mirek

Your cited reference did not look at oxidized LDL.

I believe there seems to be a relation between triglycerids,
HDL and oxLDL levels.

Anyway, further reasearch is certainly needed. Meanwhile, I
will try to stay with MUFAs as the most safe energy source🙂

Mirek